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Image Search Results
Journal: Stem Cells
Article Title: A de novo missense mutation in PPP2R5D alters dopamine pathways and morphology of iPSC-derived midbrain neurons
doi: 10.1093/stmcls/sxae068
Figure Lengend Snippet: Top DEGs are not rescued by PspdCas13b-ADAR2DD constructs and sgRNA 190. Relative expression of the 3 top DEGs between the isogenic and E198K NSC cell states in isogenic, not-treated E198K NSCs, E198K NSCs treated with Psp dCas13b-ADAR2DD(E488Q), Psp dCas13b-ADAR2DD(E488Q)-delta-984-1090, Psp dCas13b-ADAR2DD(E488Q/T375G), or Psp dCas13b-ADAR2DD(E488Q/T375G)-delta-984-1090. (A) RWDD28 relative expression determined by RT-qPCR. * Significantly different from total isogenic, one-way ANOVA P < .05. (B) ZNF717 relative expression determined by RT-qPCR. * Significantly different from total isogenic, one-way ANOVA P < .05. (C) CTSF relative expression determined by RT-qPCR. * Significantly different from total isogenic, one-way ANOVA P < .05.
Article Snippet: Four available Psp dCas13b-ADAR2DD constructs were screened:
Techniques: Construct, Expressing, Quantitative RT-PCR